Hair follicle labeled - In the K15CreER(G)T2/R26R mouse model, hair follicle stem cells (HFSCs) are specifically labeled after Cre activation upon treatment of mice with tamoxifen. By analyzing the skin tissue at different time points following genetic labeling, important information on stem cell behavior and contribution of labeled stem cells to epidermal …

 
Furthermore, therapeutic agents that target distinct phases and hormones involved in the hair cycle may be useful for promoting hair growth. Read the full article here. J Drugs Dermatol. 2014;13(suppl 1):s12-s16. Test your knowledge! Which part of the hair follicle is the first to cornify? A. Huxley’s layer of inner root sheath. What is jso

Hair follicles. Sweat glands. Collagen bundles. Fibroblasts. Nerves. Sebaceous glands. The dermis is held together by a protein called collagen. This layer gives skin flexibility and strength. The dermis also contains pain and touch receptors. Subcutaneous fat layer. The subcutaneous fat layer is the deepest layer of skin.Jun 1, 2022 · Furthermore, in pluripotent stem cell induction studies, hair follicles were produced that grew radially outward the hair-bearing skin organoids (8,9). However, despite the promise of HF organoid research, few studies have investigated the complete structure and properties of HF organoids. The hair follicle is one of only two structures within the adult body that selectively degenerates and regenerates, making it an intriguing organ to study and use for regenerative medicine. ... It was first identified in murine hair follicles as a slow cycling and thus label retaining population 19. In human hair follicles, bulge cells are ... The hair follicle is an organ found in mammalian skin. It resides in the dermal layer of the skin and is made up of 20 different cell types, each with distinct functions. The hair follicle regulates hair growth via a complex interaction between hormones, neuropeptides, and immune cells. Hair follicle is an appendage from the vertebrate skin epithelium, and is critical for environmental sensing, animal appearance, and body heat maintenance. ... Label-retaining cells reside in the bulge area of pilosebaceous unit: implications for follicular stem cells, hair cycle, and skin carcinogenesis. Cell. 1990; 61:1329–37. [Google ...The hair follicle undergoes cyclical rounds of quiescence (telogen), growth (anagen) and regression (catagen), and consists of heterogeneous compartments (labeled A–E).Label the following: Hair follicle, Sebaceous gland, Epidermis, Dermis (papillary layer), Dermis (reticular layer), Hypodermis, Arrector pili muscle, Sweat gland. Oil gland (produces sebum) Subout Epidermis Dermis. Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...Start studying Label the Hair Follicle. Learn vocabulary, terms, and more with flashcards, games, and other study tools.In healthy hair follicles, TLR2 is expressed in a cycle-dependent manner and governs HFSCs activation by countering inhibitory BMP signaling. Hair follicles in aging and obesity exhibit a decrease in both TLR2 and its endogenous ligand carboxyethylpyrrole (CEP), a metabolite of polyunsaturated fatty acids. Administration of CEP stimulates hair ...Follicular unit excision (FUE) is a donor hair harvesting technique used by hair transplant surgeons to harvest individual follicular units (tiny bundles of hair …Hair primarily serves for protection, warmth, and sensation. Human hair is made of keratin. The structure of the hair follicle includes the papilla, matrix, root, and bulb. The different types of hair on the human body include lanugo, vellus hair, and terminal hair. Attached to the follicle is a tiny bundle of muscle fiber called the arrector pili.Sebaceous glands are associated with hair follicles. The complex of hair follicle, hair shaft, and sebaceous gland is sometimes called the pilosebaceous apparatus. Histologically, sebaceous glands are quite different from all other glands. They are holocrine glands, which means that the whole cell is secreted.The cells in all of the layers except the stratum basale are called keratinocytes. A keratinocyte is a cell that manufactures and stores the protein keratin. Keratin is an intracellular fibrous protein that gives hair, nails, and skin their hardness and water-resistant properties.The keratinocytes in the stratum corneum are dead and regularly slough …Hair follicles, which house the differentiated cells of the hair shaft, ... Whole-mount K79 tm2a/+ telogen skin from 8 week old mice, showing labeled hair canals. K. β-gal activity is absent from the lower bulb of an Anagen V-VI follicle (dotted line), consistent with loss of K79. Right, magnified view of lower follicle.Telogen. This is the resting phase, which lasts roughly three months. After a few months, hair stops growing and detaches from the hair follicle. New hair starts to grow and pushes the old, dead hair out. …The hair follicle is considered a main reservoir of cutaneous stem cells, containing several pools of epithelial, melanocyte, and mesenchymal stem cells involved in hair follicle self-regeneration and pigmentation. In addition, while some of the different follicular cell types contribute to hair shaft growth, others participate in very ...The scalp is obviously hairy and has many sebaceous glands (oil glands) scattered across it. This density at which these glands are found means that the scalp can commonly be affected by sebaceous cysts. Scalp hairs protrude from structures known as hair follicles, which are situated in the dermis of the scalp.The hair follicle is encased by the dermal sheath, which appears as a fine eosinophilic line surrounding the basophilic hair follicle. Higher magnification will reveal the finite structures of the root sheath layer and hair shaft.May 1, 2023 · Hair follicles and their keratinized product, hair, are skin appendages present on nearly every part of the body. Areas of the body typically devoid of hair include the palmar and plantar surfaces, lips, and urogenital orifices. Sex hormones influence the distribution, texture, and color of hair. Hair follicles and hair are both multifunctional. Hair follicles generate hair and help to provide ... An alcohol hair test can detect whether a person has consumed alcohol in recent months. Blood and urine tests are used for the same purpose, but their timeframe is limited to a few days or hours ...labeled unlabeled. Sebaceous gland may slightly enlarge relative to the size of the hair follicle. ... The stem cell bulge and arrector pili muscles remain attached to the hair follicle cluster. This is a distinguishing factor from androgenic alopecia (where detachment of the arrector pili can occur) and scarring alopecias (where destruction of ...hair bulb that has detached from the hair matrix during the catagen phase; appears as a club-shaped mass. terminal hair. usually long and pigmented. vellus hair. very fine and usually not pigmented ("peach fuzz" hair) arrector pili muscle. attached to hair follicle and pulls it, causing goose bumps or hair to rise.The hair follicle is an organ found in mammalian skin. It resides in the dermal layer of the skin and is made up of 20 different cell types, each with distinct functions. The hair follicle regulates hair growth via a complex interaction between hormones, neuropeptides, and immune cells.Label the Hair Follicle — Quiz Information. This is an online quiz called Label the Hair Follicle. You can use it as Label the Hair Follicle practice, completely free to play.This study examined the effects of adult bulge hair follicle stem cells (HFSC) in wound healing. Materials and methods: Hair follicle stem cells were obtained from rat vibrissa and labeled with DiI (Invitrogen, Carlsbad, CA), then special markers were detected using flow cytometry. A full-thickness excisional wound model was created and DiI ...This video was produced to help students of human anatomy at Modesto Junior College study our anatomical models.Hair Shaft. The hair shaft is the solitary part of the hair follicle that fully exits the surface of the skin. The hair shaft is made up of three layers: the medulla, cortex, and the cuticle. The cuticle is the hair’s outer protective layer and is connected to the internal root sheath. It is a complex structure with a single molecular layer ...Thin skin with cross-sections of hair follicles and their associated sebaceous glands. Hair Shaft - cortex and medulla of keratinized cells. Cuticle - squamous cells form the outermost layer of hair. Internal Root Sheath - extends from the hair bulb to the level of sebaceous glands. Huxley's Layer - single or double layer of flattened cells.May 7, 2020 ... Hair follicle has hair bulb. Hair bulb contains papilla. Papilla ... Resting phase ( telogen ): Over months, hair growth stops and the old hair ...Aug 3, 2023 · The 3 innermost layers of epithelial cells within the hair follicle keratinize to produce the hair shaft. The outer epithelial layers form the hair’s epithelial sheath. The mass of cells from which the hair shaft is produced is referred to as the hair matrix. When a hair is actually growing, epithelial cells around the dermal papilla multiply. We thus developed a ROSA26 reporter line expressing photo-convertible fluorescent protein (nuclear Kikume Green–Red (nKikGR)) 15, which enabled us to photo-label target hair follicles under the ...Hair follicle is an appendage from the vertebrate skin epithelium, and is critical for environmental sensing, animal appearance, and body heat maintenance. ... Label-retaining cells reside in the bulge area of pilosebaceous unit: implications for follicular stem cells, hair cycle, and skin carcinogenesis. Cell. 1990; 61:1329–37. [Google ...The hair bulb is the lowest expanded extremity of the hair follicle that fits like a cap over the dermal hair papilla, enclosing it.The dermal hair papilla is a cluster of mesenchymal cells giving rise to …Subsequently, the surviving label-retaining cells in the hair germ migrated upward to re-epithelialize the damaged portion. These results indicate that follicular stem cells in the epithelial sac underwent cell death after plucking. It is likely that the hair germ is responsible for the reconstruction of the stem cell region of the hair follicle.Anagen is the longest phase of hair growth. It can last years for the hairs on your head, while hairs on other areas of the body tend to have shorter anagen periods. During the second phase, catagen, hair growth slows down. Cell division stops, blood flow is cut off, and a “club hair” is formed as the follicle prepares to enter its resting ...Henan Rebecca Hair Products News: This is the News-site for the company Henan Rebecca Hair Products on Markets Insider Indices Commodities Currencies StocksThe hair follicle is divided into three segments: the infundibulum, the isthmus and the inferior portion.. The hair bulb comprises the expanded portion of the inferior hair follicle and contains the dermal papilla and hair matrix. The dermal papilla consists of mesenchymal cells which function in the regulation of hair growth. This connective tissue …Hair primarily serves for protection, warmth, and sensation. Human hair is made of keratin. The structure of the hair follicle includes the papilla, matrix, root, and bulb. The different types of hair on the human body include lanugo, vellus hair, and terminal hair. Attached to the follicle is a tiny bundle of muscle fiber called the arrector pili.Hair Follicle. The slide shows a cross-section of a hair follicle. Basal cells of the hair matrix in the center differentiate into cells of the inner root sheath. Basal cells at the base of the hair root form the outer root sheath. LM × 4. (credit: modification of work by “kilbad”/Wikimedia Commons).The structural, or pilosebaceous, unit of a hair follicle consists of the hair follicle itself with an attached sebaceous gland and arrector pili muscle. The hair follicle begins at the surface of the epidermis. For follicles that produce terminal hairs, the hair follicle extends into the deep dermis, and sometimes even subcutis.Hair : Diagram of Hair Follicle. The different layers of the hair follicle. ...EPIDERMAL LAYERS. & Physiology Lab Homework by Laird C. Sheldahl, under a Creative Commons Attribution-ShareAlike License 4.0. Lab 4 Exercise 4.2.1 4.2. 1. Integument Layers. Label the following: *Hair follicle * Sebaceous gland * Epidermis * Dermis (papillary layer) *Dermis (reticular layer) * Hypodermis * Arrector pili muscle * Sweat gland. 1.The hair follicle is one of only two structures within the adult body that selectively degenerates and regenerates, making it an intriguing organ to study and use for regenerative medicine. ... It was first identified in murine hair follicles as a slow cycling and thus label retaining population 19. In human hair follicles, bulge cells are ...A photograph of hairs on a human arm, with each hair based in a hair follicle. A hair follicle is a part of the skin, which grows a hair by packing old cells together. Attached inside the top of the follicle are sebaceous glands, which are tiny sebum -producing glands in almost all skin except on the palms, lips and soles of the feet.Categories: Illustrations/Clip-Art , Science. 424 million images. Find Human Hair Follicle Labeled stock images in HD and millions of other royalty-free stock photos, illustrations and vectors in the Shutterstock collection. Thousands …The Growth Cycle. The hair on your scalp grows less than half a millimeter a day. The individual hairs are always in one of three stages of growth: anagen, catagen, and telogen. Stage 1: The anagen phase is the growth phase of the hair. Most hair spends several years in this stage.Hair follicles are essentially small cavities in your skin. Each follicle typically produces a single strand of hair (though some people may have some hair follicles that produce two or more hairs at a time). Your hair is made up of dead cells – but your hair follicles are alive! They go through repeated cycles of growth, shrinking, and rest ...The hair follicle is encased by the dermal sheath, which appears as a fine eosinophilic line surrounding the basophilic hair follicle. Higher magnification will reveal the finite structures of the root sheath …Feb 20, 2024 · Label the Heart. Medicine. English. Creator. LMaggieO +1. Quiz Type. Image Quiz. Value. 21 points. ... Hair Follicle — Quiz Information. This is an online quiz ... Jan 19, 2024 ... Learn more · Open App. Anatomy/Histology of the hair follicle wall and hair root. 149 views · 3 months ago ...more. Dr.A Explains Anatomy. 770.Start studying Label the structures of the hair. Learn vocabulary, terms, and more with flashcards, games, and other study tools.human skin. sebaceous gland. ovarian follicle. laser hair removal. sweat. epidermi. of 16. Find Hair Follicle Diagram stock images in HD and millions of other royalty-free stock photos, illustrations and vectors in the Shutterstock collection. Thousands of new, high-quality pictures added every day.1. Bulb. The bulb is found at the root of your hair where the protein cells (keratin) grow to make hair. 2. Papilla. The papilla provides blood supply to the hair follicles for healthy hair. 3. Germinal Matrix. Germinal matrix is the region where the cells produce new hairs.Label the Hair Follicle — Quiz Information. This is an online quiz called Label the Hair Follicle. You can use it as Label the Hair Follicle practice, completely free to play. hair matrix. actively dividing area of the hair bulb that produces the hair. connective tissue root sheath. derived from dermis but a bit denser, surrounds epithelial root sheath. epithelial root sheath. Extension of the epidermis lying adjacent to hair root. Widens at deep end into bulge—source of stem cells for follicle growth. While some have argued that human hair is a vestigial evolutionary remnant, in reality, human hair serves many psychological and physiological functions. Human hair grows at a rate of 0.35 mm/day, and around 100 hairs are shed daily. Human hair angiogenesis begins at about ten weeks of gestation, and final development results in …Cut the hair specimen into 1-2 cm long and have them ready on hand. 2. Brush a fingernail-sized area with clear nail polish on a blank microscope slide. Note: Latex (for molding) can be used in place of nail polish. 3. Before the nail polish is dried, quickly place the piece of hair onto the nail polish area. 4.Nov 29, 2019 - Illustration about Human Hair Anatomy. Diagram of a hair follicle and cross section of the skin layers. Illustration of dermatology, care, cuticle - 83837459What is true about apocrine sweat glands? -they are located predominantly in axillary and genital areas. -they produce clear perspiration consisting primarily of water and salts. -they are important in temperature regulation. -they are distributed all over the body. corneum, lucidum, granulosum, spinosum, basale.Hair follicles. Hair follicles are tubular invaginations lined by stratified squamous epithelium similar to epidermis. Toward the bottom of each follicle, processes of cell division, growth, and maturation similar to those in the epidermis yield a cylindrical column of dead, keratinized cells (the hair shaft) which gradually extrudes from the ...The maintenance of self-renewing tissues, including the epidermis and hair follicle, largely depends on stem cells. The follicular stem cells can be identified primarily as label retaining cells (LRC), representing their long-lived, slow-cycling nature in vivo.It was initially demonstrated that LRC are exclusively found in the bulge region that marks the …Skin that has four layers of cells is referred to as “thin skin.”. From deep to superficial, these layers are the stratum basale, stratum spinosum, stratum granulosum, and stratum corneum. Most of the skin can be classified as thin skin. “Thick skin” is found only on the palms of the hands and the soles of the feet.Custom labels are an ideal way to get organized, but it can be difficult to find something that best suits your purposes as well as your own personal design sense. Everything you n...Yes, alcohol can show up on a hair follicle test. It is important to remember that a hair follicle test is able to detect the presence of alcohol in a person’s system for up to 90 days. However, it is important to note that the amount of alcohol that can be detected in a person’s system decreases over time. Therefore, a hair follicle test ...The mesenchymal components of the hair follicle—the dermal papilla (DP) and dermal sheath (DS)—are maintained by hair follicle dermal stem cells, but the position of this stem cell population throughout the hair cycle, its contribution to the maintenance of the dermis, and the existence of a migratory axis from the DP to the dermis remain …I tried to show you all the important features from the shaft and the follicles in the dog hair labeled diagram. Rabbit hair under a microscope. The rabbit hair is extremely used in felted fabrics, gloves linings, fur trim, coats, and others. You will find the fine diameter in the hair shaft of a rabbit.Are you tired of constantly shaving or waxing unwanted hair? If so, you may have heard about Nood IPL, the latest hair removal solution on the market. Nood IPL stands for Intense P...Subsequently, the surviving label-retaining cells in the hair germ migrated upward to re-epithelialize the damaged portion. These results indicate that follicular stem cells in the epithelial sac underwent cell death after plucking. It is likely that the hair germ is responsible for the reconstruction of the stem cell region of the hair follicle.In the K15CreER(G)T2/R26R mouse model, hair follicle stem cells (HFSCs) are specifically labeled after Cre activation upon treatment of mice with tamoxifen. By analyzing the skin tissue at different time points following genetic labeling, important information on stem cell behavior and contribution of labeled stem cells to epidermal … Download 990 Hair Follicle Diagram Stock Illustrations, Vectors & Clipart for FREE or amazingly low rates! New users enjoy 60% OFF. 240,184,374 stock photos online. The hair follicle (HF) has emerged into the forefront as one of the most illustrative and accessible models to examine adult stem cell regulation in mammals, attributed to its ability to predictably cycle through well-characterized phases of degeneration (catagen), rest (telogen) and regeneration (anagen) throughout life.ブラジル1部リーグ、サンベントFCコーチングスタッフ用トレーニングシャツです。大変貴重なブラジル現地購入、日本未発売モデルとなります。It was first identified in murine hair follicles as a slow cycling and thus label retaining population 19. In human hair follicles, bulge cells are Krt15+ 20 , and possess the abilities to self-renew and differentiate into multiple cell types, fulfilling the criteria for designation as multipotent stem cells.Subsequently, the surviving label-retaining cells in the hair germ migrated upward to re-epithelialize the damaged portion. These results indicate that follicular stem cells in the epithelial sac underwent cell death after plucking. It is likely that the hair germ is responsible for the reconstruction of the stem cell region of the hair follicle.The structural, or pilosebaceous, unit of a hair follicle consists of the hair follicle itself with an attached sebaceous gland and …The hair follicle can be divided into 3 regions: the lower segment (bulb and suprabulb), the middle segment (isthmus), and the upper segment (infundibulum). The …Hair primarily serves for protection, warmth, and sensation. Human hair is made of keratin. The structure of the hair follicle includes the papilla, matrix, root, and bulb. The different types of hair on the human body include lanugo, vellus hair, and terminal hair. Attached to the follicle is a tiny bundle of muscle fiber called the arrector pili.Synonyms: Scapus pili. The hair shaft is the visible, nongrowing portion of a hair protruding from the skin. This part of the hair is not anchored to the hair follicle. The hair shaft has three layers: a central medulla, a keratinised cortex and an outer layer, known as the cuticle, which is highly keratinised and forms the thin hard cuticle on ...We thus developed a ROSA26 reporter line expressing photo-convertible fluorescent protein (nuclear Kikume Green–Red (nKikGR)) 15, which enabled us to photo-label target hair follicles under the ...Figure 13.4.1 13.4. 1: dyed hair. Hair is a filament that grows from a hair follicle in the dermis of the skin. It consists mainly of tightly packed, keratin-filled cells called keratinocytes. The human body is covered with hair follicles except for a few areas, including the mucous membranes, lips, palms of the hands, and soles of the feet.

The hair follicle has been known, since 1990, to contain stem cells located in the bulge area. In 2003, we reported a new type of stem cell in the hair follicle that expresses the brain stem-cell marker nestin. We have termed these cells as hair-follicle-associated pluripotent (HAP) stem cells. HAP stem cells can differentiate into neuronal and .... Frank's meat and produce photos

hair follicle labeled

The hair follicle is an organ found in mammalian skin. It resides in the dermal layer of the skin and is made up of 20 different cell types, each with distinct functions. The hair follicle regulates hair growth via a complex interaction between hormones, neuropeptides, and immune cells.Furthermore, therapeutic agents that target distinct phases and hormones involved in the hair cycle may be useful for promoting hair growth. Read the full article here. J Drugs Dermatol. 2014;13(suppl 1):s12-s16. Test your knowledge! Which part of the hair follicle is the first to cornify? A. Huxley’s layer of inner root sheathHenan Rebecca Hair Products News: This is the News-site for the company Henan Rebecca Hair Products on Markets Insider Indices Commodities Currencies StocksThe correct labelling is given below - Explanation - The skin is the outermost organ of our body and its main function is the protection of the inner organs. The skin has three di …. Label the following: Hair follicle * Sebaceous gland * Epidermis * Dermis (papillary layer) * Dermis (reticular layer) * Hypodermis * Arrector pili muscle ...While some have argued that human hair is a vestigial evolutionary remnant, in reality, human hair serves many psychological and physiological functions. Human hair grows at a rate of 0.35 mm/day, and around 100 hairs are shed daily. Human hair angiogenesis begins at about ten weeks of gestation, and final development results in …The dermis is a connective tissue layer sandwiched between the epidermis and subcutaneous tissue. The dermis is a fibrous structure composed of collagen, elastic tissue, and other extracellular components that includes vasculature, nerve endings, hair follicles, and glands. The role of the dermis is to support and protect the skin and …MH 086b Scalp. Only thin skin contains hair follicles and their associated sebaceous glands. - shafts (which are absent in most follicles) are found at the center of hair follicles. The. contains a cortex and medulla surrounded by a thin cuticle (light pink). - extends from the hair bulb to the level of sebaceous glands.Anagen is the longest phase of hair growth. It can last years for the hairs on your head, while hairs on other areas of the body tend to have shorter anagen periods. During the second phase, catagen, hair growth slows down. Cell division stops, blood flow is cut off, and a “club hair” is formed as the follicle prepares to enter its resting ... Abstract. Nearly 50 years ago, Chase published a review of hair cycling in which he detailed hair growth in the mouse and integrated hair biology with the biology of his day. In this review we have used Chase as our model and tried to put the adult hair follicle growth cycle in perspective. We have tried to sketch the adult hair follicle cycle ... The hair shaft is the visible, nongrowing portion of a hair protruding from the skin.This part of the hair is not anchored to the hair follicle.. The hair shaft has three layers: a central medulla, a keratinised cortex and an outer layer, known as the cuticle, which is highly keratinised and forms the thin hard cuticle on the outside of the hair.. …hair follicle: [noun] the tubular epithelial sheath that surrounds the lower part of the hair shaft and encloses at the bottom a vascular papilla supplying the growing basal part of the hair with nourishment — see hair illustration.1. Bulb. The bulb is found at the root of your hair where the protein cells (keratin) grow to make hair. 2. Papilla. The papilla provides blood supply to the hair follicles for healthy hair. 3. Germinal Matrix. Germinal matrix is the region where the cells produce new hairs.Start studying Label the Hair Follicle. Learn vocabulary, terms, and more with flashcards, games, and other study tools.Both hair distribution as well as the function of the cutaneous glands, responds to the hormonal changes associated with puberty. Areas once devoid of hair in the pre-pubertal years (axilla, pubs, chest, abdomen, and beard region in males), may become populated with active hair follicles during adolescence. Apocrine gland activity is also ...Hair is a component of the integumentary system and extends downward into the dermal layer, where it sits in the hair follicle. Humans have approximately five million hair follicles, which offer protection from cold and UV radiation, produce sebum, and can have a significant psychological impact when growth or structure is unbalanced.[1](c) Labeled bulge cells persist in the bulge in the subsequent telogen stage after the lower hair follicle regresses (30 d after RU486 treatment, left panel). Representative examples of labeled ...Search from thousands of royalty-free Hair Diagram stock images and video for your next project. Download royalty-free stock photos, vectors, HD footage and more on Adobe Stock.Jan 17, 2023 · Hair primarily serves for protection, warmth, and sensation. Human hair is made of keratin. The structure of the hair follicle includes the papilla, matrix, root, and bulb. The different types of hair on the human body include lanugo, vellus hair, and terminal hair. Attached to the follicle is a tiny bundle of muscle fiber called the arrector pili. No label-retaining cells were found in the hair canal, sebaceous gland, or hair germ. These label-retaining cells remained in the follicle following induction of anagen by plucking of the hairs. Surprisingly, they were not part of the first wave of mitotic activity following plucking, but instead underwent mitosis beginning 42 h after plucking.The cells in all of the layers except the stratum basale are called keratinocytes. A keratinocyte is a cell that manufactures and stores the protein keratin. Keratin is an intracellular fibrous protein that gives hair, nails, and skin their hardness and water-resistant properties.The keratinocytes in the stratum corneum are dead and regularly slough ….

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